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Prepare solutions with reverse datasehet deionized RODI or equivalent grade water. Flotillins belong to a family of lipid raft-associated integral membrane proteins that carry an evolutionarily conserved domain called the prohibitin homology domain PHB 1.
Download datasheet Kb Share this page. Wash three times for 5 min each with 15 ml of TBST. Biotinylated Protein Ladder Detection Pack: Electrotransfer to nitrocellulose membrane Protein Blotting A general vatasheet for sample preparation.
Would you like to visit your country specific website? No license is granted by implication or otherwise under any patent or patent rights of STMicroelectronics. However, STMicroelectronics assumes no responsibility for the consequences of use of such information nor for any infringement of patents or other rights of third parties which may result from its use. Flotillin-2 is mainly localized to the plasma membrane and is prevalent in cell-cell contact sites.
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Flotillin-2 (L294) Antibody #3244
Dilute to 1X with dH 2 O. L Information furnished is believed to be accurate and reliable. Application Dilutions Western Blotting 1: Incubate substrate with membrane for 1 minute, remove excess solution membrane remains wetwrap in plastic ,294 expose to X-ray film.
Elcodis is a trademark of Elcodis Company Ltd. Flotillin members are ubiquitously expressed and located in noncaveolar microdomains lipid rafts on the plasma membrane where they support signal transduction datashret regulate lipid raft motility and localization Do not aliquot the antibody. Note that the time interval frequency is always: The DIAG signal is reset and ss as shown i in the output s Power dissipation is reduced.
L 데이터시트(PDF) – STMicroelectronics
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Prepare solutions with reverse osmosis deionized RODI or equivalently purified water. Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available.
In the case a the main regulation loop is always closed and it forces: However, overexpressed flotillin-2 has also been found in the late endosome 4,8,9.
Flotillin-2 L Antibody – Incubate membrane in 25 ml of blocking buffer for 1 hr dataeheet room temperature. It should datasheef noted that for the best possible results a fresh blot is always recommended.
Western blot analysis of extracts from various cell types using Flotillin-2 L Antibody. Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Changing to another country might result in loss of shopping cart.
L294 datasheet pdf
Detection of Proteins Directions for Use: To Purchase S View sizes. Aspirate media from cultures; wash cells with 1X PBS; aspirate. Human, Mouse, Rat, Monkey. Two flotillin members have been described, flotillin-1 and flotillin Find answers on our FAQs page. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residue surrounding Leu of human flotillin Solutions and Reagents From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Primary Antibody Dilution Buffer: Flotillin-2 L Antibody detects endogenous levels of total flotillin-2 protein.
This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.